LAP3 ELISA Kit (Human) (OKCD06433)

Catalog No: OKCD06433

Size:96WELLS

LAP3 ELISA Kit (Human) (OKCD06433) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Aminopeptidase (LAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Aminopeptidase (LAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Aminopeptidase (LAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leucine Aminopeptidase (LAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Aminopeptidase (LAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Aminopeptidase (LAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • LAP3 Microplate: 96 Wells (12 x 8 Well strips)
  • LAP3 Lyophilized Standard: 2
  • 100X LAP3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.065ng/mL

Target Info

• Gene Symbol: LAP3
• Gene Full Name: leucine aminopeptidase 3
• Alias Symbols: cysteinylglycine-S-conjugate dipeptidase;cytosol aminopeptidase;epididymis secretory protein Li 106;HEL-S-106;LAP;LAPEP;Leucine aminopeptidase 3;leucyl aminopeptidase;PEPS;peptidase S;proline aminopeptidase;prolyl aminopeptidase.
• NCBI Gene Id: 51056
• Protein Name: Cytosol aminopeptidase
• Description of Target: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
• Uniprot ID: P28838
• Protein Accession #: NP_056991.2
• Nucleotide Accession #: NM_015907.2