LAMTOR1 ELISA Kit (Human) (OKWB00202)

Catalog No: OKWB00202

Size:96T

Human LAMTOR1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	85-105%	87-99%	94-102%	87-100%
  EDTA Plasma (n=5)	90-95%	83-98%	86-100%	86-97%
  Heparin Plasma (n=5)	80-98%	81-100%	91-100%	82-94%

• ELISA Kit Principle: Aviva Systems Biology LAMTOR1 ELISA Kit (Human) (OKWB00202) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAMTOR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAMTOR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAMTOR1 captured in the well.
• ELISA Kit Range: 0.313-20ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	86-103	95
  EDTA Plasma (n=5)	85-99	90
  Heparin Plasma (n=5)	86-104	95

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • LAMTOR1 Microplate: 96 Wells (12 x 8 Well strips)
  • LAMTOR1 Lyophilized Standard: 2 x 20 ng
  • 100X LAMTOR1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.188 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LAMTOR1
• Gene Full Name: late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
• Alias Symbols: C11orf59;late endosomal/lysosomal adaptor and MAPK and MTOR activator 1;lipid raft adaptor protein p18;p18;p27kip1 releasing factor from RhoA;p27Kip1-releasing factor from RhoA;p27RF-Rho;PDRO;protein associated with DRMs and endosomes;ragulator complex protein LAMTOR1;ragulator complex protein PDRO;Ragulator1;RhoA activator C11orf59.
• NCBI Gene Id: 55004
• Protein Name: Ragulator complex protein LAMTOR1
• Description of Target: As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation.
• Uniprot ID: Q6IAA8
• Protein Accession #: NP_060377
• Nucleotide Accession #: NM_017907