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Datasheets/Manuals | Printable datasheet for OASE00061-Biotin |
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Predicted Species Reactivity | Mouse, Rabbit |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Monoclonal |
Clone | GL2A7 |
Isotype | IgG1 |
Host | Rat |
Conjugation | Biotin |
Application | IP, ICC, IF |
Additional Information | Background Info: Recommended for use in ICC. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin. |
:: | Scientific Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C 9$) and prostaglandin D 9@), which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7). |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Purified preparation of mouse liver lysosomal membranes |
Purification | Protein G Purified |
Concentration | 1 mg/ml |
Dilution | 1:500- 1:1000 (ICC) |
Storage Buffer | PBS, 50% glycerol |
Reference | 1. Granger B.L., et al. (1990) J. Biol. Chem. 265: 12036-12043. 2. Furuta K., et al. (1999) EMBO J. 17(5):1304-14. 3. Rohrer J., et al. (1996) J Cell Biol. 132(4): 565-76. 4. Lichter-Konecki U., et al (1999) Differentiation 65(1): 43-58. 5. Grutzkau A., et al. (2004) Cytometry A. 61(1): 62-68. 6. Kannan K., et al. (1996) Cell Immunol. 171: 10-19. 7. Tanaka Y., et al. (2000) Nature 406: 902-906. |
Gene Symbol | Lamp2 |
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Gene Full Name | lysosomal-associated membrane protein 2 |
Alias Symbols | Lam, Mac, Lamp, Mac3, LGP-B, CD107b, Lamp-2, Lamp II, Lamp-2a, Lamp-2b, Lamp-2c |
NCBI Gene Id | 16784 |
Uniprot ID | P17047 |
Protein Accession # | NP_001017959.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "LAMP2 Antibody : Biotin (OASE00061-Biotin)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse, Rabbit".
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How long will it take to receive "LAMP2 Antibody : Biotin (OASE00061-Biotin)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "LAMP2 Antibody : Biotin (OASE00061-Biotin)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "LAMP2 Antibody : Biotin (OASE00061-Biotin)"?
This target may also be called "Lam, Mac, Lamp, Mac3, LGP-B, CD107b, Lamp-2, Lamp II, Lamp-2a, Lamp-2b, Lamp-2c" in publications.
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What is the shipping cost for "LAMP2 Antibody : Biotin (OASE00061-Biotin)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "LAMP2 Antibody : Biotin (OASE00061-Biotin)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "LAMP2 Antibody : Biotin (OASE00061-Biotin)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "LAMP2 Antibody : Biotin (OASE00061-Biotin)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "LAMP2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "LAMP2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "LAMP2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "LAMP2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "LAMP2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "LAMP2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.