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Catalog No: OASE00058 (Formerly GWB-75AA4F)
Size:25ug
Price: $239.00
SKU
OASE00058
Availability: Discontinued
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Datasheets/ManualsPrintable datasheet for OASE00058
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Hamster
ClonalityMonoclonal
CloneLy1C6
IsotypeIgG1
HostMouse
ConjugationUnconjugated
ApplicationWB, ICC, IF, IP
Additional InformationBackground Info: Detects an ~120kDa protein corresponding to the molecular mass of LAMP1 on SDS PAGE immunoblots.
::Scientific Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3).
This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).
::Certificate of Analysis: 1ug/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.
Reconstitution and StorageStore at -20C. Shipping with Blue Ice or 4C.
ImmunogenRat liver lysosomal membrane preparations
PurificationProtein G Purified
Concentration1 mg/ml
SpecificityDetects ~120kDa.
DilutionWB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Storage BufferPBS pH7.4, 50% glycerol, 0.09% sodium azide
Reference1. Grutzkau, A. et al (2004) Cytometry A. 61(10): 62-68.
2. Furuta, K. et al. (1999) EMBO J. 17(5):1304-14.
3. Rohrer, J. et al. (1996) J Cell Biol. 132(4): 565-76.
4. Kannan, K., et al. (1996) Cell Immunol. 171: 10-19.
5. Lewis, V., et al. (1985) J. Cell Biol. 100: 1839-1847.
6. Jones, K.A., et al. (2004) Exp Cell Res 295(2): 512-524.
DescriptionLysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).
Gene SymbolLAMP1
Gene Full Namelysosomal-associated membrane protein 1
Alias SymbolsCD107, CD107a, LAMPA, LGP120, IgpA, Lysosome-associated membrane glycoprotein 1, CD107 antigen-like family member A, 120 kDa lysosomal membrane glycoprotein, Lamp-1
NCBI Gene Id25328
Uniprot IDP14562
Protein Accession #NP_036989.1
  1. What is the species homology for "LAMP1 Antibody (OASE00058)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Hamster".

  2. How long will it take to receive "LAMP1 Antibody (OASE00058)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "LAMP1 Antibody (OASE00058)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "LAMP1 Antibody (OASE00058)"?

    This target may also be called "CD107, CD107a, LAMPA, LGP120, IgpA, Lysosome-associated membrane glycoprotein 1, CD107 antigen-like family member A, 120 kDa lysosomal membrane glycoprotein, Lamp-1" in publications.

  5. What is the shipping cost for "LAMP1 Antibody (OASE00058)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "LAMP1 Antibody (OASE00058)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "LAMP1 Antibody (OASE00058)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "LAMP1 Antibody (OASE00058)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "LAMP1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "LAMP1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "LAMP1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "LAMP1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "LAMP1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "LAMP1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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