LAMP1 Antibody : FITC (OASE00059-FITC)

Catalog No: OASE00059-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Rat, Hamster
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: Ly1C6
• Isotype: IgG1
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: WB, IP, ICC
• Additional Information: Background Info: Detects an ~120kDa protein corresponding to the molecular mass of LAMP1 on SDS PAGE immunoblots.
  Scientific Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3).
  This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).
  
  Certificate of Analysis: 1ug/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Rat liver lysosomal membrane preparations
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1ug/ml was sufficient for detection by immunoblot analysis
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Grutzkau, A. et al (2004) Cytometry A. 61(10): 62-68.
  2. Furuta, K. et al. (1999) EMBO J. 17(5):1304-14.
  3. Rohrer, J. et al. (1996) J Cell Biol. 132(4): 565-76.
  4. Kannan, K., et al. (1996) Cell Immunol. 171: 10-19.
  5. Lewis, V., et al. (1985) J. Cell Biol. 100: 1839-1847.
  6. Jones, K.A., et al. (2004) Exp Cell Res 295(2): 512-524.

Target Info

• Gene Symbol: Lamp1
• Gene Full Name: lysosomal-associated membrane protein 1
• Alias Symbols: LGP120
• NCBI Gene Id: 25328
• Uniprot ID: P14562
• Protein Accession #: NP_036989.1