LAMA5 ELISA Kit (Mouse) (OKCD07983)

Catalog No: OKCD07983

Size:96WELLS

LAMA5 ELISA Kit (Mouse) (OKCD07983) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 5 (LAMa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 5 (LAMa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 5 (LAMa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Laminin Alpha 5 (LAMa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Alpha 5 (LAMa5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Alpha 5 (LAMa5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • LAMA5 Microplate: 96 Wells (12 x 8 Well strips)
  • LAMA5 Lyophilized Standard: 2
  • 100X LAMA5 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 33pg/mL

Target Info

• Gene Symbol: Lama5
• Gene Full Name: laminin, alpha 5
• Alias Symbols: [a]5;AA408760;AA408762;AI853660;laminin subunit alpha-5;laminin-10 subunit alpha;laminin-11 subunit alpha;laminin-15 subunit alpha;laminin-511;mKIAA0533.
• NCBI Gene Id: 16776
• Protein Name: Laminin subunit alpha-5
• Description of Target: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Alpha-5 may be the major laminin alpha chain of adult epithelial and/or endothelial basal laminae.
• Uniprot ID: Q61001
• Protein Accession #: NP_001074640.1
• Nucleotide Accession #: NM_001081171.2