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Catalog No: OKCD07981
Size:96WELLS
Price: $800.00
SKU
OKCD07981
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Datasheets/ManualsPrintable datasheet for LAMA3 ELISA Kit (Human) (OKCD07981)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 3 (LAMa3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 3 (LAMa3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 3 (LAMa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Laminin Alpha 3 (LAMa3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Alpha 3 (LAMa3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Alpha 3 (LAMa3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
LAMA3 Microplate96 Wells (12 x 8 Well strips)
LAMA3 Lyophilized Standard2
100X LAMA3 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 6.0pg/mL
Gene SymbolLAMA3
Gene Full Namelaminin subunit alpha 3
Alias SymbolsBM600;BM600 150kD subunit;E170;epiligrin 170 kda subunit;epiligrin alpha 3 subunit;Epiligrin subunit alpha;kalinin 165kD subunit;kalinin subunit alpha;LAM3, alpha-3 subunit;laminin 5, alpha-3 subunit;laminin A3;laminin subunit alpha-3;laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin);laminin-5 alpha 3 chain;laminin-5 subunit alpha;laminin-6 subunit alpha;laminin-7 subunit alpha;LAMNA;LOCS;nicein 150kD subunit;nicein subunit alpha.
NCBI Gene Id3909
Protein NameLaminin subunit alpha-3
Description of TargetBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Uniprot IDQ16787
Protein Accession #NP_000218.3
Nucleotide Accession #NM_000227.4
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