LAMA1 ELISA Kit (Rat) (OKCD00663)

Catalog No: OKCD00663 (Formerly GWB-KBBYB9)

Size:96WELLS

Rat Lama1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	93-101%	98-105%	78-92%	92-101%
  EDTA plasma(n=5)	96-103%	80-103%	82-93%	88-95%
  heparin plasma(n=5)	87-103%	91-98%	83-94%	83-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 1 (LAMA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 1 (LAMA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 1 (LAMA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Laminin Alpha 1 (LAMA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	83-96	90
  EDTA plasma(n=5)	97-105	101
  heparin plasma(n=5)	78-94	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Alpha 1 (LAMA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Alpha 1 (LAMA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Lama1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Lama1 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated Lama1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.55 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Laminin Alpha 1 (LAMa1). No significant cross-reactivity or interference between Laminin Alpha 1 (LAMa1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Lama1
• Gene Full Name: laminin subunit alpha 1
• Alias Symbols: laminin subunit alpha-1;laminin, alpha 1;laminin-1.
• NCBI Gene Id: 316758
• Protein Name: Laminin subunit alpha 1
• Description of Target: Laminin subunit alpha 1
• Uniprot ID: D4A409
• Protein Accession #: NP_001101707
• Nucleotide Accession #: NM_001108237.2
• Protein Size (# AA): 3083