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Catalog No: OKEH00147
Size:96T
Price: $525.00
SKU
OKEH00147
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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LAMA1 ELISA Kit (Human) : 96 Wells (OKEH00147)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00147
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology LAMA1 ELISA Kit (Human) (OKEH00147) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAMA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAMA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAMA1 captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 11.2% (n = 20)
ELISA Kit Component
ComponentAmount
LAMA1 Microplate96 Wells (12 x 8 Well strips)
LAMA1 Lyophilized Standard2
100X Biotinylated LAMA1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human Laminin subunit alpha-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLAMA1
Gene Full Namelaminin subunit alpha 1
Alias SymbolsLAMA;laminin A chain;laminin subunit alpha-1;laminin, alpha 1;Laminin-1 subunit alpha;laminin-3 subunit alpha;PTBHS;S-LAM alpha;S-LAM-alpha;S-laminin subunit alpha.
NCBI Gene Id284217
Protein NameP25391
Description of TargetLaminin subunit alpha-1
Uniprot IDhttps://www.uniprot.org/uniprot/P25391
Protein Accession #NP_005550
Nucleotide Accession #NM_005559
Protein Size (# AA)3075
Molecular Weight337 kDa
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