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Catalog No: OKCD07566
Size:96WELLS
Price: $750.00
SKU
OKCD07566
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Datasheets/ManualsPrintable datasheet for LAG3 ELISA Kit (Human) (OKCD07566)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Activation Gene 3 (LAG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Activation Gene 3 (LAG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Activation Gene 3 (LAG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lymphocyte Activation Gene 3 (LAG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphocyte Activation Gene 3 (LAG3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphocyte Activation Gene 3 (LAG3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
LAG3 Microplate96 Wells (12 x 8 Well strips)
LAG3 Lyophilized Standard2
100X LAG3 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.056ng/mL
Gene SymbolLAG3
Gene Full Namelymphocyte activating 3
Alias SymbolsCD223;lymphocyte activation gene 3 protein;lymphocyte-activation gene 3;Protein FDC.
NCBI Gene Id3902
Protein NameLymphocyte activation gene 3 protein
Description of TargetInvolved in lymphocyte activation. Binds to HLA class-II antigens.
Uniprot IDP18627
Protein Accession #NP_002277.4
Nucleotide Accession #NM_002286.5
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