L-FABP ELISA Kit (Rat) (OKIA00165)

Catalog No: OKIA00165 (Formerly GWB-374Z43)

Size:1x96WELLPLATE

Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 80 Minutes
• ELISA Kit Principle: Aviva Systems Biology L-FABP ELISA Kit (Rat) (OKIA00165) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Fatty acid-binding protein, liver has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Fatty acid-binding protein, liver - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fatty acid-binding protein, liver captured in the well.
• ELISA Kit Range: 3.125 ng/ml - 200 ng/ml
• ELISA Kit Component:
  • Anti-Rat L-FABP Microplate: 96 Wells (12 x 8 Well Strips)
  • 20X Diluent Concentrate: 50 mL
  • 20X Wash Solution: 50 mL
  • 100X Enzyme-Antibody Conjugate: 150 uL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
  • Rat L-FABP Calibrator: 1
• Additional Information: INTENDED USE
  The Fatty Acid Binding Protein (FABP) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring FABP in biological samples of Rat.
  REAGENTS
  1. DILUENT (Running Buffer)
  One bottle containing 60 ml of a 1X diluent running buffer.
  2. WASH SOLUTION CONCENTRATE
  One bottle containing 50 ml of a 20X concentrated wash solution.
  3. ENZYME-ANTIBODY CONJUGATE 100X
  One vial containing 150 L of affinity purified anti-Rat FABP antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  4. CHROMOGEN-SUBSTRATE SOLUTION
  One vial containing 12 mL of 3,3’,5,5’- tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  5. STOP SOLUTION
  One vial containing 12 ml 0.3 M sulfuric acid.
  WARNING: Avoid contact with skin.
  6. ANTI-RAT FABP ELISA MICRO PLATE
  Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat FABP.
  7. RAT FABP CALIBRATOR
  One vial containing Rat FABP Calibrator.
  
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 1.278 ng/ml
• Predicted Homology Based on Immunogen Sequence: Rat
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FABP1
• Gene Full Name: fatty acid binding protein 1
• Alias Symbols: SCP, p14, SCP., Fabplg, L-FABP
• NCBI Gene Id: 24360
• Protein Name: Fatty acid-binding protein, liver
• Description of Target: Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes. Binds cholesterol. Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
• Uniprot ID: P02692