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Catalog No: OKIA00165 (Formerly GWB-374Z43)
Size:1x96WELLPLATE
Price: $536.00
SKU
OKIA00165
Availability: Domestic: within 1 week delivery | International: 1 week
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration80 Minutes
ELISA Kit PrincipleAviva Systems Biology L-FABP ELISA Kit (Rat) (OKIA00165) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Fatty acid-binding protein, liver has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Fatty acid-binding protein, liver - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fatty acid-binding protein, liver captured in the well.
ELISA Kit Range3.125 ng/ml - 200 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Rat L-FABP Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat L-FABP Calibrator1
Additional InformationINTENDED USE
The Fatty Acid Binding Protein (FABP) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring FABP in biological samples of Rat.
::REAGENTS
1. DILUENT (Running Buffer)
One bottle containing 60 ml of a 1X diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 L of affinity purified anti-Rat FABP antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’- tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT FABP ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat FABP.
7. RAT FABP CALIBRATOR
One vial containing Rat FABP Calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity1.278 ng/ml
Predicted Homology Based on Immunogen SequenceRat
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFABP1
Gene Full Namefatty acid binding protein 1
Alias SymbolsSCP, p14, SCP., Fabplg, L-FABP
NCBI Gene Id24360
Protein NameFatty acid-binding protein, liver
Description of TargetPlays a role in lipoprotein-mediated cholesterol uptake in hepatocytes. Binds cholesterol. Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
Uniprot IDP02692
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