- Predicted Species Reactivity:
- Human, Mouse, Rat
- Product Format:
- Liquid. Purified antibody supplied in 1x PBS buffer.
- WB, ICC
- Additional Information:
- Background Info: ~105-125kDa (varies with cell background due to phosphorylation)
- Scientific Background: Voltage gated channels are tetrameters composed of four alpha-subunits arranged around a central pore. Each alpha- subunit consists of six transmembrane segments with cytoplasmic NH2 and COHO-termini. Members of the KV1-KV4 subfamilies generate functional K+ channels in a homotetrameric configuration. The KV2 subfamily consists of KV2.1 and KV2.2, and both have very similar properties. Members of the KV2 subfamily are widely expressed in neuronal tissues. They have also been reported in neurons in the dorsal root ganglia (3-5).
- Certificate of Analysis: 1ug/mL was sufficient for detection of Kv2.1 in 20ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Reconstitution and Storage:
- All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
- NCBI Gene Id:
- Official Gene Full Name:
- potassium voltage gated channel, Shab-related subfamily, member 1
- Protein G Purified
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express Kcnb1.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express Kcnb1.
- Synthetic peptide amino acids 11-229 (LPELQSLDEFGQSTDNPQL, extracellular S1-S2 loop) of rat Kv2.1
- 1 mg/ml
- 1ug/mL (WB), 0.1-1ug/mL (IHC/ICC), 1-10ug/mL (IF)
- Storage Buffer:
- PBS pH7.4, 50% glycerol
- Target Reference:
- 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA.
3. Bocksteins E., et al. (2009) Am J Physiol. 296:C1271-C1278.
4. Ishikawa K, Tanaka M, Black J.A., and Wasman S.G. (1999)
Muscle Nerve 22: 502-507.
5. Kim D.S., Choi J.O., Rim H.D., and Cho H.J. (2002) Brain Res
Mol Brain Res. 105: 146-152.