KTN1 ELISA Kit (Human) (OKCD01205)

Catalog No: OKCD01205 (Formerly GWB-KBBSW1)

Size:96WELLS

Human KTN1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	80-91%	89-101%	89-102%	79-95%
  EDTA plasma(n=5)	88-95%	90-99%	79-93%	81-91%
  heparin plasma(n=5)	96-104%	86-93%	98-105%	83-93%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinectin 1 (KTN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinectin 1 (KTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinectin 1 (KTN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kinectin 1 (KTN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-101	94
  EDTA plasma(n=5)	92-101	96
  heparin plasma(n=5)	96-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kinectin 1 (KTN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kinectin 1 (KTN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KTN1 Microplate: 96 Wells (12 x 8 Well Strips)
  • KTN1 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated KTN1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 30 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kinectin 1 (KTN1). No significant cross-reactivity or interference between Kinectin 1 (KTN1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: KTN1
• Gene Full Name: kinectin 1
• Alias Symbols: CG1;CG-1 antigen;kinectin;kinesin receptor;KNT;MU-RMS-40.19.
• NCBI Gene Id: 3895
• Protein Name: Kinectin
• Description of Target: Receptor for kinesin thus involved in kinesin-driven vesicle motility. Accumulates in integrin-based adhesion complexes (IAC) upon integrin aggregation by fibronectin.
• Uniprot ID: Q86UP2
• Protein Accession #: NP_001072989
• Nucleotide Accession #: NM_001079521.1
• Protein Size (# AA): 1357