KSR1 Antibody - N-terminal region (Phospho-A7) (OAAB17266)

Catalog No: OAAB17266

Size:400ul

Western blot analysis of anti-KSR1 Pab (Cat. # OAAB17266) in A375 cell lysate. KSR1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: KSR1 Antibody (N-term A7) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity
  Cellular Location: Cytoplasm. Membrane; Peripheral membrane protein (By similarity). Endoplasmic reticulum membrane. Note=In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery (By similarity)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This KSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KSR1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IHC-P: 1:50~100

Target Info

• Gene Symbol: KSR1
• Alias Symbols: KSR, RSU2
• NCBI Gene Id: 8844
• Protein Name: Kinase suppressor of Ras 1
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q8IVT5
• Protein Accession #: NP_055053.1
• Molecular Weight: 102032 Da