KRT8 Protein (OPPA01261)

Catalog No: OPPA01261 (Formerly GWB-E4DF15)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Additional Information: Reconstitution to filaments: Performed by mixing equumolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
• Reconstitution and Storage: Reconstitution: It is recommended to reconstitute the lyophilized KRT8 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Storage: Lyophilized KRT8 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution KRT8 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purification: The KRT8 is purified by proprietary chromatographic techniques.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Source: E. coli

Target Info

• Gene Symbol: KRT8
• Alias Symbols: K8, KO, CK8, CK-8, CYK8, K2C8, CARD2
• NCBI Gene Id: 3856
• Protein Name: Keratin, type II cytoskeletal 8
• Description of Target: Keratin 8 and 18 (K8/18) are the major components of intermediate filament (IF) proteins of simple or single-layered epithelia.
• Uniprot ID: P05787
• Protein Accession #: NP_002264.1
• Protein Size (# AA): Recombinant