KRT6A ELISA Kit (Human) (OKCD08433)

Catalog No: OKCD08433

Size:96WELLS

KRT6A ELISA Kit (Human) (OKCD08433) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 6A (KRT6A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 6A (KRT6A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 6A (KRT6A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Keratin 6A (KRT6A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • KRT6A Microplate: 96 Wells (12 x 8 Well strips)
  • KRT6A Lyophilized Standard: 2
  • 100X KRT6A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 6.5pg/mL

Target Info

• Gene Symbol: KRT6A
• Gene Full Name: keratin 6A
• Alias Symbols: CK6A;CK6C;CK-6C;CK6D;CK-6E;cytokeratin 6A;cytokeratin 6C;cytokeratin 6D;Cytokeratin-6A;Cytokeratin-6D;K6A;K6C;K6D;keratin 6A, , type II;keratin 6A, type II;keratin, epidermal type II, K6A;keratin, type II cytoskeletal 6A;Keratin-6A;KRT6C;KRT6D;PC3;type-II keratin Kb6.
• NCBI Gene Id: 3853
• Protein Name: Keratin, type II cytoskeletal 6A
• Description of Target: Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.
• Uniprot ID: P02538
• Protein Accession #: NP_005545.1
• Nucleotide Accession #: NM_005554.3