KRT40 ELISA Kit (Human) (OKCD09477)

Catalog No: OKCD09477

Size:96 Wells

KRT40 ELISA Kit (Human) (OKCD09477) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology KRT40 ELISA Kit (Human) (OKCD09477) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KRT40 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KRT40 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KRT40 captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level KRT40. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level KRT40 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • KRT40 Microplate: 96 Wells (12 x 8 Well strips)
  • KRT40 Lyophilized Standard: 2
  • 100X KRT40 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056ng/mL

Target Info

• Gene Symbol: KRT40
• Gene Full Name: keratin 40
• Alias Symbols: K40, KA36, CK-40
• NCBI Gene Id: 125115
• Protein Name: Keratin, type I cytoskeletal 40
• Description of Target: This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21.
• Uniprot ID: Q6A162
• Protein Accession #: NP_872303.2
• Nucleotide Accession #: NM_182497.3