KRT20 Protein (OPPA01265)

Catalog No: OPPA01265 (Formerly GWB-3BDED0)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The protein (1mg/1ml) was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM DDT, 2mM EDTA and 10mM methylammonium chloride. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Reconstitution: Performed by mixing equumolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  Solubility: It is recommended to reconstitute the lyophilized KRT20 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Product Introduction: KRT20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
  Product Description: Cytokeratin 20 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain having a molecular mass of 48,553 Dalton. The KRT20 is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized KRT20 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution KRT20 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Target Info

• Gene Symbol: KRT20
• Alias Symbols: K20, CD20, CK20, CK-20, KRT21
• NCBI Gene Id: 54474
• Protein Name: Keratin, type I cytoskeletal 20
• Description of Target: Recombinant Human Cytokeratin 20
• Uniprot ID: P35900
• Protein Accession #: NP_061883.1
• Protein Size (# AA): Recombinant