Catalog No: OKEH06174
Size:96 Wells
Price: $525.00
SKU
OKEH06174
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Krt2 ELISA Kit (Rat) (OKEH06174) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Krt2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Krt2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Krt2 captured in the well.
ELISA Kit Range0.156 - 10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 108%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <4.5%, n = 20
Mean Inter-Assay Precision - <8.2%, n = 20
ELISA Kit Component
ComponentAmount
KRT2 Microplate96 Wells (12 x 8 Well strips)
KRT2 Lyophilized Standard2
100X Biotinylated KRT2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.087 ng/mL
SpecificityNatural and recombinant Rat Keratin, type II cytoskeletal 2 epidermal
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKRT2
Gene Full NameKeratin 2
Alias SymbolsKb2, CK 2e
NCBI Gene Id406228
Protein NameKeratin, type II cytoskeletal 2 epidermal
Description of TargetProbably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization.
Swissprot IdQ6IG02
Protein Accession #NP_001008899.1
Nucleotide Accession #NM_001008899.1
Protein Size (# AA)685
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express KRT2.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express KRT2.
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