Catalog No: OKEH01005
Size:96 Wells
Price: $525.00
SKU
OKEH01005
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KRT2 ELISA Kit (Human) (OKEH01005) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KRT2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KRT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KRT2 captured in well.
ELISA Kit Range0.156 - 10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 9.7% (n = 20)
ELISA Kit Component
ComponentAmount
KRT2 Microplate96 Wells (12 x 8 Well strips)
KRT2 Lyophilized Standard2
100X Biotinylated KRT2 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Human Keratin, type II cytoskeletal 2 epidermal
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKRT2
Gene Full Namekeratin 2
Alias SymbolsK2e, KRTE, CK-2e, KRT2A, KRT2E
NCBI Gene Id3849
Protein NameKeratin, type II cytoskeletal 2 epidermal
Description of TargetThe protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Swissprot IdP35908
Protein Accession #NP_000414.2
Nucleotide Accession #NM_000423.2
Protein Size (# AA)639
Molecular Weight65 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express KRT2.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express KRT2.
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