KRT19 Chemi-Luminescent ELISA Kit (Human) (OKCD03408)

Catalog No: OKCD03408

Size:96WELLS

Human KRT19 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	97-105%	93-101%	90-98%	97-105%
  EDTA Plasma (n=5)	80-88%	92-101%	94-102%	86-94%
  Heparin Plasma (n=5)	80-95%	90-98%	89-104%	80-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	90-97	93
  Heparin Plasma (n=5)	80-90	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • KRT19 Microplate: 96 Wells (12 x 8 Well strips)
  • KRT19 Lyophilized Standard: 2
  • 100X KRT19 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.04 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: KRT19
• Gene Full Name: keratin 19
• Alias Symbols: 40-kDa keratin intermediate filament;CK19;CK-19;cytokeratin 19;Cytokeratin-19;K19;K1CS;keratin 19, type I;keratin, type I cytoskeletal 19;keratin, type I, 40-kd;Keratin-19.
• NCBI Gene Id: 3880
• Protein Name: Keratin, type I cytoskeletal 19
• Description of Target: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
• Uniprot ID: P08727
• Protein Accession #: NP_002267
• Nucleotide Accession #: NM_002276