KRT18 ELISA Kit (Rat) (OKCD02602)

Catalog No: OKCD02602

Size:96WELLS

Rat KRT18 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-101%	93-103%	86-96%	78-91%
  EDTA Plasma (n=5)	80-91%	95-104%	84-94%	81-102%
  Heparin Plasma (n=5)	95-102%	98-105%	89-103%	94-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 18 (CK18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 18 (CK18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 18 (CK18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytokeratin 18 (CK18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92-101	96
  EDTA Plasma (n=5)	80-89	85
  Heparin Plasma(n=5)	79-98	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 18 (CK18) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 18 (CK18) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KRT18 Microplate: 96 Wells (12 x 8 Well strips)
  • KRT18 Lyophilized Standard: 2
  • 100X Biotinylated KRT18 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 18 (KRT18). No significant cross-reactivity or interference between Keratin 18 (KRT18) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Krt18
• Gene Full Name: keratin 18
• Alias Symbols: CK-18;cytokeratin-18;K18;keratin 18, type I;keratin complex 1, acidic, gene 18;keratin, type I cytoskeletal 18;Keratin-18;Krt1-18.
• NCBI Gene Id: 294853
• Protein Name: Keratin, type I cytoskeletal 18
• Description of Target: When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells.
• Uniprot ID: Q5BJY9
• Protein Accession #: NP_446428.1
• Nucleotide Accession #: NM_053976.1
• Protein Size (# AA): 423