KRT14 Protein (OPPA01262)

Catalog No: OPPA01262 (Formerly GWB-D55D8E)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Reconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCI, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  Product Introduction: Cytokeratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. Cytokeratin 14 is a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
• Reconstitution and Storage: Reconstitution: It is recommended to reconstitute the lyophilized CK-14 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Storage: Lyophilized KRT14 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution KRT14 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Target Info

• Gene Symbol: KRT14
• Alias Symbols: K14, NFJ, CK14, EBS3, EBS4
• NCBI Gene Id: 3861
• Protein Name: Keratin, type I cytoskeletal 14
• Description of Target: Cytokeratin 14 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain having a molecular mass of 51,530 Dalton. The KRT14 is purified by proprietary chromatographic techniques.
• Uniprot ID: P02533
• Protein Accession #: NP_000517.2
• Protein Size (# AA): Recombinant