KRT14 ELISA Kit (Human) (OKCD00245)

Catalog No: OKCD00245 (Formerly GWB-KBBHZ5)

Size:96WELLS

Human KRT14 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	82-101%	78-89%	89-105%	93-105%
  EDTA plasma(n=5)	89-97%	80-95%	90-99%	78-88%
  heparin plasma(n=5)	88-96%	83-105%	86-98%	87-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 14 (CK14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 14 (CK14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 14 (CK14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytokeratin 14 (CK14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	97-105	101
  EDTA plasma(n=5)	92-105	101
  heparin plasma(n=5)	78-98	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 14 (CK14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 14 (CK14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KRT14 Microplate: 96 Wells (12 x 8 Well Strips)
  • KRT14 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated KRT14 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 14 (KRT14). No significant cross-reactivity or interference between Keratin 14 (KRT14) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: KRT14
• Gene Full Name: keratin 14
• Alias Symbols: CK14;cytokeratin 14;Cytokeratin-14;EBS3;EBS4;K14;keratin 14, type I;keratin, type I cytoskeletal 14;Keratin-14;NFJ.
• NCBI Gene Id: 3861
• Protein Name: Keratin, type I cytoskeletal 14
• Description of Target: The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
• Uniprot ID: P02533
• Protein Accession #: NP_000517
• Nucleotide Accession #: NM_000526.4
• Protein Size (# AA): 472