KRT14 Antibody (OAAF01841)

Catalog No: OAAF01841 (Formerly GWB-ASD112)

Size:100 ug

Western blot analysis of extracts from NIH-3T3 cells, using Keratin 14 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Keratin 14 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using Keratin 14 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Keratin 14.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Keratin 14 antibody detects endogenous levels of total Keratin 14 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Reference: Nam-On Ku, PNAS, Apr 2002; 99: 4373.
  E L Rugg, Genes & Dev., Nov 1994; 8: 2563 - 2573.
  Julia Reichelt, Mol. Biol. Cell, Jun 2001; 12: 1557.
  P. E. Bowden, Cancer Res., Nov 1992; 52: 5865 - 5871.

Target Info

• Gene Symbol: KRT14|KRT16|KRT17
• Gene Full Name: keratin 14|keratin 16|keratin 17
• Alias Symbols: 39.1;CK14;CK16;CK-17;cytokeratin 14;cytokeratin 16;Cytokeratin-14;Cytokeratin-16;cytokeratin-17;EBS3;EBS4;FNEPPK;focal non-epidermolytic palmoplantar keratoderma;K14;K16;K17;K1CP;keratin 14, type I;keratin 16, type I;keratin 17, type I;keratin, type I cytoskeletal 14;keratin, type I cytoskeletal 16;keratin, type I cytoskeletal 17;Keratin-14;Keratin-16;KRT16A;NEPPK;NFJ;PC;PC1;PC2;PCHC1.
• NCBI Gene Id: 3861|3868|3872
• Protein Name: Keratin, type I cytoskeletal 14|Keratin, type I cytoskeletal 16
• Description of Target: Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.|The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
• Uniprot ID: P02533|P08779
• Protein Accession #: NP_000517.2
• Molecular Weight: 51 kDa