KRT13 ELISA Kit (Human) (OKEH07026)

Catalog No: OKEH07026

Size:96T

Human KRT13 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology KRT13 ELISA Kit (Human) (OKEH07026) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-KRT13 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated KRT13 and incubated. The KRT13 found in the sample or standards competes with the biotinylated KRT13 for limited binding sites on the immobilized anti-KRT13 antibody. Excess unbound biotinylated KRT13 and sample or standard KRT13 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated KRT13 captured in the well and inversely proportional to the amount of KRT13 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 98%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <5.6%
  Inter-Assay CV <9.8%
• ELISA Kit Component:
  • KRT13 Microplate: 96 Wells (12 x 8 Well strips)
  • KRT13 Lyophilized Standard: 2 x 20 ng
  • 100X KRT13 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.15 ng/mL
• Specificity: Natural and recombinant Human Keratin, type I cytoskeletal 13
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: KRT13
• Gene Full Name: keratin 13
• Alias Symbols: CK13;CK-13;cytokeratin 13;Cytokeratin-13;K13;keratin 13, type I;keratin, type I cytoskeletal 13;Keratin-13;WSN2.
• NCBI Gene Id: 3860
• Protein Name: P13646
• Description of Target: Keratin, type I cytoskeletal 13
• Uniprot ID: https://www.uniprot.org/uniprot/P13646
• Protein Accession #: NP_002265
• Nucleotide Accession #: NM_002274