KRT13 Antibody (OAGA03556)

Catalog No: OAGA03556

Size:100 ul

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded CAL27 xenograft, using Cytokeratin 13 antibody at 1:500 dilution.

Immunofluorescence analysis of methanol-fixed MCF-7, using Cytokeratin 13 antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Cytokeratin 13. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.91 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: KRT13
• Gene Full Name: keratin 13
• Alias Symbols: K13, CK13, WSN2
• NCBI Gene Id: 3860
• Protein Name: keratin, type I cytoskeletal 13
• Description of Target: The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described.
• Uniprot ID: P13646
• Protein Accession #: NP_002265.2
• Nucleotide Accession #: NM_002274.3