Catalog No: OKCD07595
Size:96WELLS
Price: $750.00
SKU
OKCD07595
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Datasheets/ManualsPrintable datasheet for KRT10 ELISA Kit (Human) (OKCD07595)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 10 (CK10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 10 (CK10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 10 (CK10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytokeratin 10 (CK10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 10 (CK10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 10 (CK10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
KRT10 Microplate96 Wells (12 x 8 Well strips)
KRT10 Lyophilized Standard2
100X KRT10 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 11.7pg/mL
Gene SymbolKRT10
Gene Full Namekeratin 10
Alias SymbolsBCIE;BIE;CK10;CK-10;cytokeratin 10;Cytokeratin-10;EHK;K10;keratin 10, type I;keratin, type I cytoskeletal 10;Keratin-10;KPP.
NCBI Gene Id3858
Protein NameKeratin, type I cytoskeletal 10
Description of TargetPlays a role in the establishment of the epidermal barrier on plantar skin.
Uniprot IDP13645
Protein Accession #NP_000412.3
Nucleotide Accession #NM_000421.3
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