KRT1 ELISA Kit (Rat) (OKCD00244)

Catalog No: OKCD00244 (Formerly GWB-KBBTN2)

Size:96WELLS

Rat Krt1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	80-95%	89-97%	83-90%	83-94%
  EDTA plasma(n=5)	91-99%	92-105%	84-93%	82-90%
  heparin plasma(n=5)	79-95%	99-105%	83-90%	89-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 1 (CK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 1 (CK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 1 (CK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytokeratin 1 (CK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	96-105	101
  EDTA plasma(n=5)	98-105	102
  heparin plasma(n=5)	81-90	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 1 (CK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 1 (CK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Krt1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Krt1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Krt1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 1 (KRT1). No significant cross-reactivity or interference between Keratin 1 (KRT1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Krt1
• Gene Full Name: keratin 1
• Alias Symbols: CK-1;cytokeratin-1;K1;Kb1;keratin 1, type II;keratin, type II cytoskeletal 1;Keratin-1;type-II keratin Kb1.
• NCBI Gene Id: 300250
• Protein Name: Keratin, type II cytoskeletal 1
• Description of Target: May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK (By similarity).
• Uniprot ID: Q6IMF3
• Protein Accession #: NP_001008802
• Nucleotide Accession #: NM_001008802.2
• Protein Size (# AA): 625