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Catalog No: OCOA07112
Size:20UG
Price: $179.00
SKU
OCOA07112
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KRAS Protein Lysate (OCOA07112)

Datasheets/ManualsPrintable datasheet for KRAS Protein Lysate (OCOA07112)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionKRAS HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolKRAS
Gene Full NameKRAS proto-oncogene, GTPase
Alias Symbolscellular c-Ki-ras2 proto-oncogene;cellular transforming proto-oncogene;CFC2;c-Ki-ras;c-Ki-ras2;c-Kirsten-ras protein;C-K-RAS;'C-K-RAS;GTPase KRas;KI-RAS;Kirsten rat sarcoma viral oncogene homolog;Kirsten rat sarcoma viral proto-oncogene;K-Ras;K-Ras 2;K-ras p21 protein;KRAS1;KRAS2;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;NS;NS3;OES;oncogene KRAS2;PR310 c-K-ras oncogene;RALD;RASK2;transforming protein p21;v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog.
NCBI Gene Id3845
Protein NameI1SRC5, L7RSL8, NM_033360, NP_203524, P01116
Description of TargetGTPase KRas, GTPase KRas isoform a [Homo sapiens], Homo sapiens KRAS proto-oncogene, GTPase (KRAS), transcript variant a, mRNA, UBE2L3/KRAS fusion protein, V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, isoform CRA_a
Uniprot IDhttps://www.uniprot.org/uniprot/P01116
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_203524
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_033360
Molecular Weight21.5 kDa
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