KRAS ELISA Kit (Human) (OKCD01039)

Catalog No: OKCD01039 (Formerly GWB-KBBBO5)

Size:96WELLS

Human KRAS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	81-103%	82-90%	90-98%	88-95%
  EDTA plasma(n=5)	90-98%	94-101%	93-101%	99-105%
  heparin plasma(n=5)	88-101%	80-95%	79-102%	91-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	89-97	92
  EDTA plasma(n=5)	78-97	87
  heparin plasma(n=5)	99-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KRAS Microplate: 96 Wells (12 x 8 Well Strips)
  • KRAS Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated KRAS Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS). No significant cross-reactivity or interference between V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: KRAS
• Gene Full Name: KRAS proto-oncogene, GTPase
• Alias Symbols: cellular c-Ki-ras2 proto-oncogene;cellular transforming proto-oncogene;CFC2;c-Ki-ras;c-Ki-ras2;c-Kirsten-ras protein;C-K-RAS;'C-K-RAS;GTPase KRas;KI-RAS;Kirsten rat sarcoma viral oncogene homolog;Kirsten rat sarcoma viral proto-oncogene;K-Ras;K-Ras 2;K-ras p21 protein;KRAS1;KRAS2;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;NS;NS3;OES;oncogene KRAS2;PR310 c-K-ras oncogene;RALD;RASK2;transforming protein p21;v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog.
• NCBI Gene Id: 3845
• Protein Name: GTPase KRas
• Description of Target: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:20949621). Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306).
• Uniprot ID: P01116
• Protein Accession #: NP_004976
• Nucleotide Accession #: NM_004985.4
• Protein Size (# AA): 189