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KRAS Antibody (OACD05037)

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Predicted Species Reactivity:
Human
Product Format:
Liquid
Clonality:
Polyclonal
Isotype:
IgG
Host:
Rabbit
Application:
WB, ICC, IHC-P, IHC-F, ELISA
Reconstitution and Storage:
Store at +4C. For long term storage, aliquot and store at -20C. Avoid freeze/thaw cycles.
Official Gene Full Name:
KRAS proto-oncogene, GTPase
NCBI Gene Id:
3845
Protein Name:
GTPase KRas
Swissprot Id:
P01116
Protein Accession #:
NP_004976.2
Nucleotide Accession #:
NM_004985
Alias Symbols:
c-Ki-ras, c-K-ras, C-K-RAS, GTPase KRas, Ki-Ras, KI-RAS, KRAS1, KRAS2, K-Ras 2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, NS, NS3, RASK2
Description of Target:
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner.Curated
Molecular Weight:
21 kDa
Purification:
Antigen-specific affinity chromatography
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express KRAS.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express KRAS.
Immunogen:
Range: Met1 - Met189
Concentration:
200 ug/ml
Datasheets/Manuals:
Printable datasheet for anti-KRAS (OACD05037) antibody
Preservative:
0.02% NaN3
Specificity:
The antibody is a rabbit polyclonal antibody raised against KRAS. It has been selected for its ability to recognize KRAS in immunohistochemical staining and western blotting.
Formulation:
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol
Application Data:
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5,000
Optimal working dilutions must be determined by end user.

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