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Catalog No: OKEH04634
Size:96T
Price: $525.00
SKU
OKEH04634
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology KPNA2 ELISA Kit (Human) (OKEH04634) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KPNA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for KPNA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KPNA2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-KPNA2 Microplate96 Wells (12 x 8 Well Strips)
KPNA2 Lyophilized Standard2
Sample Diluent1 x 20 mL
KPNA2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.21 ng/mL
SpecificityNatural and recombinant Human Importin subunit alpha-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKPNA2
Gene Full Namekaryopherin subunit alpha 2
Alias Symbolsimportin subunit alpha-1;importin subunit alpha-2;importin-alpha-P1;IPOA1;karyopherin alpha 2 (RAG cohort 1, importin alpha 1);Karyopherin subunit alpha-2;pendulin;QIP2;RAG cohort protein 1;RCH1;SRP1alpha;SRP1-alpha.
NCBI Gene Id3838
Protein NameP52292
Description of TargetImportin subunit alpha-1
Uniprot IDhttps://www.uniprot.org/uniprot/P52292
Protein Accession #NP_001307540.1
Nucleotide Accession #NM_001320611.1
Protein Size (# AA)529
Molecular Weight58 KDa
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