Selecting Your Primary Antibody

Selecting Your Primary Antibody

When selecting antibodies for your research, there are several key factors to consider.

Here are some guidelines to help you make an informed decision, but always feel free to reach out to Aviva’s Tech Support for more help!

Research your target: Consider the specific target molecule or protein you are studying. Review its biological function, tissue and cellular localization, and any known variations in isoforms or sequences. Some antibodies recognize and bind to specific regions of the protein, and this knowledge will help you choose an antibody that best recognizes your target of interest.

Read product specifications thoroughly: Carefully review the product specifications provided. Look for information about the antibody's specificity, sensitivity, and recommended applications. Pay attention to the host species, the antigen used for immunization, and any validation data provided.

Review the antibody validation data: Check if the antibody has been validated for the application(s) and species relevant to your research. Look for publications or customer reviews that report successful results using the antibody in similar experiments or systems. Validation information provides confidence in the antibody's performance, and Aviva highlights those with Enhanced Validation qualities, using our Enhanced Validation Icon

Evaluate experimental requirements: Assess your experimental requirements, such as the technique you plan to use (e.g., Western blot, immunofluorescence, immunohistochemistry), the sample type (e.g., cell lysates, tissue sections), and the species you are working with. Ensure that the antibody is compatible with your specific experimental conditions, but keep in mind that antibodies not yet validated for specific applications or species may still perform well and should be validated by the end used in their samples.

Seek expert advice: If you are uncertain or require further guidance, consult with experts in your field, colleagues, or email Aviva’s technical support team! We can provide valuable insights and recommendations based on our QC data and previous experience.

Check return policies: Before making a purchase, familiarize yourself with any supplier's return policy in case the antibody does not perform as expected. This information will give you peace of mind and flexibility if you encounter any issues. Aviva aims to provide the highest quality reagents and the best customer experience. Aviva guarantees antibody performance as described in each of our datasheets.

By considering these factors and conducting thorough research, you can make an informed decision and select antibodies that are most likely to yield reliable and accurate results in your experiments.