KNG1 Antibody (OAGA00072)

Catalog No: OAGA00072

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
10% SDS PAGE
OAGA00072 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Kininogen 1(OAGA00072) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Kininogen-1(OAGA00072) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7) with 0.01% Thimerosal was added as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, ICC, IF, IHC-P
• Additional Information: Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
• Reconstitution and Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HMW Kininogen. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: WB: 1:500-1:3000
  ICC: 1:100-1:1000
  IF: 1:100-1:1000
  IHC-P: 1:100-1:1000
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: KNG1
• Gene Full Name: Kininogen-1
• Alias Symbols: BK, BDK, KNG, HMWK
• NCBI Gene Id: 3827
• Protein Name: Kininogen-1
• Description of Target: High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein (see MIM 147910)-kinin system. The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing. Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3. However, HMWK contains a 56-kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4-kD light chain that consists of domain 5L. In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide. BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin (see MIM 601699) from endothelial cells. During vascular damage, BK stimulates smooth muscle proliferation and intimal hypertrophy. Release of BK from HMWK generates a 2-chain HMWK, termed HMWKa, containing the heavy and light chains joined by a disulfide bond.
• Uniprot ID: P01042
• Protein Accession #: NP_000884.1
• Molecular Weight: 72 kDa