KLRB1 Antibody : PerCP-Cy5.5 (OATA00498)

Catalog No: OATA00498

Human peripheral blood lymphocytes were stained with 5 uL (0.5 ug) PerCP-Cy5.5 Anti-Human CD161 (OATA00498) (solid line) or 0.5 ug PerCP-Cy5.5 Mouse IgG1 isotype control (dashed line).

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Chimpanzee, Baboon, Rhesus
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: HP-3G10
• Isotype: Mouse IgG1, kappa
• Host: Mouse
• Conjugation: PERCP: Peridinin Chlorophyll Protein Complex, CY5.5: Cyanine 5.5
• Application: FC
  Excitation Laser: Blue (488 nm)
  Emmision: 676 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD161
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:

  Yamada H, Nakashima Y, Okazaki K, Mawatari T, Fukushi J-I, Oyamada A, Fujimura K, Iwamoto Y, and Yoshikai Y. 2011. J. Rheumatol. 38: 1569-1575. (Flow cytometry)

  Fogal B, Yi T, Wang C, Rao DA, Lebastchi A, Kulkarni S, Tellides G, and Pober JS. 2011. J. Immunol. 187: 6268-6280. (in vitro depletion)

  Pozo, D, Vales-Gomez, Mavaddat N, Williamson SC, Chisholm SE, and Reyburn H. 2006. J. Immunol. 176: 2397-2406. (Western blot)

  Exley M, Porcelli S, Furman M, Garcia J, and Balk S. 1998. J. Exp. Med. 188: 867-876. (in vitro blocking, Western blot)

Target Info

• Gene Symbol: KLRB1
• Gene Full Name: killer cell lectin like receptor B1
• Alias Symbols: NKR, CD161, CLEC5B, NKR-P1, NKRP1A, NKR-P1A, hNKR-P1A
• NCBI Gene Id: 3820
• Protein Name: killer cell lectin-like receptor subfamily B member 1
• Description of Target: Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus.
• Uniprot ID: Q12918
• Protein Accession #: NP_002249.1
• Nucleotide Accession #: NM_002258.2