KLKB1 ELISA Kit (Rat) (OKCA00991)

Catalog No: OKCA00991

Size:96 Wells

Rat KLKB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  1:200	91	86-95
  1:400	103	99-105
  1:800	91	85-97
  1:1600	96	91-99

• ELISA Kit Principle: Aviva Systems Biology Klkb1 ELISA Kit (Rat) (OKCA00991) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Klkb1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Klkb1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Klkb1 captured in well.
• ELISA Kit Range: 7.8 ng/mL-500 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95	89-98
  EDTA Plasma (n=4)	97	95-99

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Klkb1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Klkb1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Klkb1 Microplate: 96 Wells (12 x 8 Well strips)
  • Klkb1 Lyophilized Standard: 2
  • 100X Biotinylated Klkb1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.95 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: KLKB1
• Gene Full Name: Plasma kallikrein
• Alias Symbols: Fletcher factor, Kalp1, KALP15, Kininogenin, Klk3, MGC108748, Pk, Plasma kallikrein, Plasma prekallikrein
• Protein Name: Plasma kallikrein
• Description of Target: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
• Uniprot ID: P14272
• Protein Accession #: NP_036857.2
• Nucleotide Accession #: NM_012725.2