KLK9 ELISA Kit (Rat) (OKEH03108)

Catalog No: OKEH03108

Size:96T

Rat KLK9 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: ELISA-Comp
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology KLK9 ELISA Kit (Rat) (OKEH03108) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Klk9 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Klk9 and incubated. The Klk9 found in the sample or standards competes with the biotinylated Klk9 for limited binding sites on the immobilized anti-rat Klk9 antibody. Excess unbound biotinylated Klk9 and sample or standard Klk9 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Klk9 captured in the well and inversely proportional to the amount of Klk9 which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • Anti-Klk9 Microplate: 96 Wells (12 x 8 Well strips)
  • Klk9 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Klk9-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.085 ng/mL
• Specificity: Natural and recombinant Rat Submandibular glandular kallikrein-9
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Klk1c9
• Gene Full Name: kallikrein 1-related peptidase C9
• Alias Symbols: kallikrein a2;kallikrein, submaxillary gland S3;Klk9;Klks3;KLK-S3;Klna2;rGK-9;rK9;S3 kallikrein;SEV;submandibular enzymatic vasoconstrictor;submandibular glandular kallikrein-9;submaxillary gland S3 kallikrein;tissue kallikrein.
• NCBI Gene Id: 292868
• Protein Name: P07647
• Description of Target: Submandibular glandular kallikrein-9
• Uniprot ID: https://www.uniprot.org/uniprot/P07647
• Protein Accession #: NP_786935.1
• Nucleotide Accession #: NM_175759.2
• Protein Size (# AA): 259
• Molecular Weight: 28 kDa