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Catalog No: OKEH03192
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KLK8 ELISA Kit (Rat) (OKEH03192) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Klk8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Klk8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Klk8 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Component
Anti-Klk8 Microplate96 Wells (12 x 8 Well strips)
Klk8 Lyophilized Standard2
100X Biotinylated Klk8 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.33 ng/mL
SpecificityNatural and recombinant Rat Kallikrein-8
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKLK8
Gene Full Namekallikrein related-peptidase 8
Alias SymbolsBrain serine protease 1;Neuropsin;Serine protease 19.
NCBI Gene Id308565
Protein NameO88780
Description of TargetKallikrein-8
Uniprot ID
Protein Accession #NP_001100979.1 
Nucleotide Accession #NM_001107509.1
Protein Size (# AA)260
Molecular Weight29 kDa
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