KLK6 Polyclonal Antibody (OABB00719)

Catalog No: OABB00719

Size:100UG

Lane 1: MCF-7 Cell Lyste
Lane 2: HELA Cell Lyste
Lane 3: M231 Cell Lyste
Lane 4: M453 Cell Lyste
Lane 5: A549 Cell Lyste
Lane 6: SMMC Cell Lyste
Lane 7: COLO320 Cell Lyste
Lane 8: SW620 Cell Lyste
Lane 9: HT1080 Cell Lyste

IHC(P):Human Intestinal Cancer Tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunocytochemistry|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: KLK6(Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Immunocytochemistry : 0.5-1 ug/ml: Human
• Reference: 1. Anisowicz, A., Sotiropoulou, G., Stenman, G., Mok, S. C., Sager, R. A novel protease homolog
  differentially expressed in breast and ovarian cancer. Molec. Med. 2: 624-636, 1996.
  2. Elliott, M. B., Irwin, D. M., Diamandis, E. P. In silico identification and Bayesian phylogenetic analysis
  of multiple new mammalian kallikrein gene families. Genomics 88: 591-599, 2006.
  3. Iwata, A., Maruyama, M., Akagi, T., Hashikawa, T., Kanazawa, I., Tsuji, S., Nukina,
  N. Alpha-synuclein degradation by serine protease neurosin: implication for pathogenesis of
  synucleinopathies.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Kallikrein-6(KLK6) detection. Tested with WB, IHC-P, ICC in Human.

Target Info

• Gene Symbol: KLK6
• Gene Full Name: kallikrein related peptidase 6
• Alias Symbols: Bssp;hK6;kallikrein-6;Klk7;neurosin;protease M;PRSS18;PRSS9;serine protease 18;serine protease 9;SP59;zyme.
• NCBI Gene Id: 5653
• Protein Name: Kallikrein-6
• Description of Target: Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.
• Uniprot ID: Q92876
• Molecular Weight: 26856 MW