Size:96 Wells
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KLK3 ELISA Kit (Human) : 96 Wells (OKEH00249)

Catalog#: OKEH00249
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology KLK3 ELISA Kit (Human) (OKEH00249) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KLK3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for KLK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KLK3 captured in well.
Kit Range 0.156 - 10ng/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Kit Reproducibility Mean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
Kit Component
KLK3 Microplate96 Wells (12 x 8 Well strips)
KLK3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated KLK3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 0.078 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Human Prostate-specific antigen
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol KLK3
Official Gene Full Name kallikrein related peptidase 3
Alias Symbols APS, Gamma-seminoprotein, hK3, Kallikrein-3, KLK2A1, P-30 antigen, Prostate-specific antigen, PSA, Semenogelase, Seminin
NCBI Gene Id 354
Protein Name Prostate-specific antigen
Description of Target Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Swissprot Id P07288
Protein Accession # NP_001025218.1
Nucleotide Accession # NM_001030047.1
Protein Size (# AA) 261
Molecular Weight 29 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express KLK3.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express KLK3.
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