KLK3 Chemi-Luminescent ELISA Kit (Human) (OKCD03705)

Catalog No: OKCD03705

Size:96WELLS

Human KLK3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-103%	99-105%	91-99%	79-101%
  EDTA Plasma (n=5)	83-90%	80-104%	84-99%	92-105%
  Heparin Plasma (n=5)	88-95%	96-103%	78-102%	95-103%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Prostate Specific Antigen (PSA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Prostate Specific Antigen (PSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Prostate Specific Antigen (PSA) level in the sample or standard.
• ELISA Kit Range: 54.9-40,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92-101	95
  EDTA Plasma (n=5)	80-102	91
  Heparin Plasma (n=5)	85-99	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostate Specific Antigen (PSA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostate Specific Antigen (PSA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • KLK3 Microplate: 96 Wells (12 x 8 Well strips)
  • KLK3 Lyophilized Standard: 2
  • 100X KLK3 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.0549 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: KLK3
• Gene Full Name: kallikrein related peptidase 3
• Alias Symbols: APS;gamma-seminoprotein;hK3;kallikrein-3;KLK2A1;P-30 antigen;prostate-specific antigen;PSA;semenogelase;seminin.
• NCBI Gene Id: 354
• Protein Name: Prostate-specific antigen
• Description of Target: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
• Uniprot ID: P07288
• Protein Accession #: NP_001025218
• Nucleotide Accession #: NM_001030047