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KLK1B3 ELISA Kit (Rat) (OKCD04999)

  • Catalog#: OKCD04999
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Application:
ELISA
Gene Symbol:
KLK1B3
Official Gene Full Name:
kallikrein 1-related peptidase B3
NCBI Gene Id:
24594
Alias Symbols:
Klk1, Ngfg, rK-1, Klna4, rGK-1, Klk1c1
Description of Target:
a putative pancreatic kallikrein.
Protein Name:
kallikrein 1-related peptidase B3
Swissprot Id:
P00758
Protein Accession #:
NM_031523.1
Nucleotide Accession #:
NP_113711.1
Predicted Species Reactivity:
Rat
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
< 48.2pg/mL
Kit Range:
123.5-10000pg/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level KLK1B3. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level KLK1B3 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology KLK1B3 ELISA Kit (Rat) (OKCD04999) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KLK1B3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KLK1B3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KLK1B3 captured in the well.
Kit Component:
ComponentAmount
KLK1B3 Microplate96 Wells (12 x 8 Well strips)
KLK1B3 Lyophilized Standard2
100X KLK1B3 HRP-Detector Anitbody1 x 120 µL
100X Avidin-HRP Conjugate1 x 120 µL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by RNA-seq analysis to express KLK1B3.
RNA Seq:
Find tissues and cell lines supported by DNA array analysis to express KLK1B3.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.

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