Catalog No: OKCD00155 (Formerly GWB-KBBUT1)
Size:96WELLS
Price: $625.00
SKU
OKCD00155
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Datasheets/ManualsPrintable datasheet for KLK1B3 ELISA Kit (Mouse) (OKCD00155)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)83-104%87-96%88-99%94-101%
EDTA plasma(n=5)83-98%87-102%81-91%85-96%
heparin plasma(n=5)79-96%78-95%86-101%84-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostate Specific Antigen (PSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostate Specific Antigen (PSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostate Specific Antigen (PSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prostate Specific Antigen (PSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)90-104101
EDTA plasma(n=5)80-9287
heparin plasma(n=5)79-10388
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostate Specific Antigen (PSA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostate Specific Antigen (PSA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Klk1b3 Microplate96 Wells (12 x 8 Well Strips)
Klk1b3 Lyophilized Standard2 x 1 ng
100X Biotinylated Klk1b3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 6.1 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Kallikrein 3 (KLK3). No significant cross-reactivity or interference between Kallikrein 3 (KLK3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolKlk1b3
Gene Full Namekallikrein 1-related peptidase b3
Alias Symbols7S nerve growth factor gamma chain;7S nerve growth factor gamma subunit;gamma-NGF;glandular kallikrein K3;kallikrein 1-related peptidase b3;mGk-3;Ngf;Ngfg;tissue kallikrein-3.
NCBI Gene Id18050
Protein NameKallikrein 1-related peptidase b3
Description of Target7S NGF alpha chain stabilizes the 7S complex. The beta dimer promotes neurite growth. The gamma chain is an arginine-specific protease; it may also have plasminogen activator activity, as well as mitogenic activity for chick embryo fibroblasts.
Uniprot IDP00756
Protein Accession #NP_032719
Nucleotide Accession #NM_008693.2
Protein Size (# AA)261
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