Catalog No: OKCD00765 (Formerly GWB-KBBYZ9)
Size:96WELLS
Price: $675.00
SKU
OKCD00765
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Datasheets/ManualsPrintable datasheet for KLK13 ELISA Kit (Human) (OKCD00765)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)89-98%80-94%93-101%88-102%
EDTA plasma(n=5)98-105%94-102%92-101%79-101%
heparin plasma(n=5)95-102%91-103%86-95%78-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 13 (KLK13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 13 (KLK13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 13 (KLK13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kallikrein 13 (KLK13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)87-10295
EDTA plasma(n=5)84-10197
heparin plasma(n=5)93-10198
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kallikrein 13 (KLK13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kallikrein 13 (KLK13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-KLK13 Microplate96 Wells (12 x 8 Well Strips)
KLK13 Lyophilized Standard2 x 2 ng
100X Biotinylated KLK13 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 12.6 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Kallikrein 13 (KLK13). No significant cross-reactivity or interference between Kallikrein 13 (KLK13) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolKLK13
Gene Full Namekallikrein related peptidase 13
Alias Symbolskallikrein-13;kallikrein-like gene 4;kallikrein-like protein 4;KLKL4;KLK-L4.
NCBI Gene Id26085
Protein NameKallikrein-13
Description of TargetKallikrein-13
Uniprot IDQ9UKR3
Protein Accession #NP_056411
Nucleotide Accession #NM_015596.1
Protein Size (# AA)277
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