Size:96 Wells
$469.00
In stock
SKU
OKEH03256
Request Bulk
Order Quote
Aviva's
Satisfaction
Guarantee
Contact Us:
  • Toll Free: (888)880-0001
  • Phone: (858)552-6979
  • Email: info@avivasysbio.com
Shipping Info:
  • $40: Antibody & Protein in US
  • $50: 1-2 Kits in US
  • Contact us for international orders.

KLK1 ELISA Kit (Mouse) (OKEH03256)

Catalog#: OKEH03256
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology KLK1 ELISA Kit (Mouse) (OKEH03256) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Klk1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Klk1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Klk1 captured in well.
Kit Range 0.156 - 10ng/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
Kit Reproducibility Mean Intra-assay CV%: <= 7.4% (n = 20)
Mean Inter-assay CV%: <= 9.4% (n = 20)
Kit Component
ComponentAmount
Anti-Klk1 Microplate96 Wells (12 x 8 Well strips)
Klk1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Klk1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 0.083 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse Kallikrein-1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol KLK1
Official Gene Full Name kallikrein 1
Alias Symbols 0610007D04Rik, Glandular kallikrein K1, Kal, KAL-B, Kallikrein-1, Klk1b6, Klk6, Klk-6, mGk-6, mGK-6, Renal kallikrein, Tissue kallikrein-6
NCBI Gene Id 16612
Protein Name Kallikrein-1
Description of Target Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Swissprot Id P15947
Protein Accession # NP_001307260.1
Nucleotide Accession # NM_001320331.1
Protein Size (# AA) 261
Molecular Weight 29 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Klk1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Klk1.
Write Your Own Review
You're reviewing:KLK1 ELISA Kit (Mouse) (OKEH03256)
Your Rating