KLK1 ELISA Kit (Mouse) (OKCD06675)

Catalog No: OKCD06675

Size:96WELLS

KLK1 ELISA Kit (Mouse) (OKCD06675) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 6 (KLK6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 6 (KLK6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 6 (KLK6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kallikrein 6 (KLK6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kallikrein 6 (KLK6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kallikrein 6 (KLK6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • KLK1 Microplate: 96 Wells (12 x 8 Well strips)
  • KLK1 Lyophilized Standard: 2
  • 100X KLK1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, cerebrospinal fluid, breast milk and other biological fluids
• Sensitivity: < 13.4pg/mL

Target Info

• Gene Symbol: Klk6
• Gene Full Name: kallikrein related-peptidase 6
• Alias Symbols: AI849898;B;brain serine protease;BSP;Bssp;kallikrein 29;kallikrein 6;kallikrein-6;Klk2;Klk29;MSP;myelencephalon-specific protease;myofibril-bound protease;ne;neurosin;P;Pr;protease M;protease, serine, 18;protease, serine, 9;Prss18;Prss9.
• NCBI Gene Id: 19144
• Protein Name: Kallikrein 6, isoform CRA_a
• Description of Target: Kallikrein 6, isoform CRA_a
• Uniprot ID: P15947
• Protein Accession #: NP_001307260.1
• Nucleotide Accession #: NM_001320331.1