KLK1 ELISA Kit (Human) (OKBB00359)

Catalog No: OKBB00359 (Formerly GWB-F015Y7)

Size:96 Tests

Human KLK1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human KLK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK1 has been precoated onto 96-well plates. Standards(NS0, I25-S262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human KLK1 amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	1468	3882	6352	1625	4190	6526
  St. Dev.	67.53	260.1	349.4	95.9	301.7	417.7
  %CV	4.60	6.70	5.50	5.90	7.20	6.40

• ELISA Kit Component:
  • Lyophilized recombinant human P53 standard: 10 ng/tube x 2
  • Anti-human P53 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human P53 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 156pg/ml-10,000pg/ml
  Principle: Aviva’s human KLK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human KLK1 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK1 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
• Sensitivity: <10 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Richards RI, Holman K, Shen Y, Kozman H, Harley H, Brook D, Shaw D (Feb 1992). "Human glandular Kallikrein
  genes: genetic and physical mapping of the KLK1 locus using a highly polymorphic microsatellite PCR marker". Genomics
  11 (1): 77–82. Doi: 10.1016/0888-7543(91)90103-L. PMID 1684954.
  2. Lundwall A, Band V, Blaber M, Clements JA, Courty Y, Diamandis EP, Fritz H, Lilja H, Malm J, Maltais LJ, Olsson AY,
  Petraki C, Scorilas A, Sotiropoulou G, Stenman UH, Stephan C, Talieri M, Yousef GM (Jun 2006). "A comprehensive
  nomenclature for serine proteases with homology to tissue kallikreins". Biol Chem 387 (6): 637–41.
  doi:10.1515/BC.2006.082. PMID 16800724.
  3. Diamandis, Eleftherios P.; Deperthes, David; Lundwall, Åke (Jun 2006). "Proceedings of the 1st International
  Symposium on Kallikreins, Lausanne, Switzerland, September 1-3, 2005". Biol Chem 387 (6): 635–824.
  doi:10.1515/BC.2006.081. PMID 16800723.
• Specificity: Natural and recombinant human KLK1
• Immunogen: Expression system for standard: NS0; Immunogen sequence: I25-S262
• Description: For quantitative detection of human KLK1 in cell culture supernatants, cell lysates, serum and plasma(heparin, EDTA).

Target Info

• Gene Symbol: KLK1
• Gene Full Name: kallikrein 1
• Alias Symbols: Glandular kallikrein 1, hK1, Kallikrein 1 renal/pancreas/salivary, Kallikrein serine protease 1, Kallikrein-1, Kidney/pancreas/salivary gland kallikrein, KLK1, KLK1_HUMAN, Klk6, KLKR, Tissue kallikrein
• NCBI Gene Id: 3816
• Protein Name: Kallikrein-1
• Description of Target: Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
• Uniprot ID: P06870
• Protein Accession #: NP_002248.1
• Nucleotide Accession #: NM_002257.3