KLF4 Antibody (OAAD00376)

Catalog No: OAAD00376

Size:0.1ml

western blot analysis using KLF4 mAb against HEK293 (1) and KLF4-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and lung cancer tissues (right) using KLF4 mouse mAb with DAB staining

Immunofluorescence analysis of ECA109 cells using KLF4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: 1E6
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IF
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Immunogen: Purified recombinant fragment of human KLF4 expressed in E. Coli.
• Formulation: Ascitic fluid containing 0.03% sodium azide.
• Application Info: Western Bloting: 1/500 - 1/2000.
  Immunohistochemistry: 1/200 - 1/1000.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: Propose dilution 1/10000.
  Not yet tested in other applications.
  Determining optimal working dilutions by titration test.
• Publications:

  Luz-Madrigal, A. et al. Reprogramming of the chick retinal pigmented epithelium after retinal injury. BMC Biol. 12, 28 (2014). 24742279

• Reference: 1. Mol Cell Proteomics. 2008 Mar;7(3):499-508.
  2. J Cancer Res Clin Oncol. 2008 Aug;134(8):891-8.

Target Info

• Gene Symbol: KLF4
• Alias Symbols: EZF, GKLF
• NCBI Gene Id: 9314
• Description of Target: Kruppel-like factor 4 (gut), endothelial Kruppel-like zinc finger protein. May act as a transcriptional activator. Binds the CACCC core sequence. May be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 is highly expressed in the epithelial cells of the skin and the gastrointestinal tract.
• Uniprot ID: O43474
• Molecular Weight: 54kDa