Catalog No: OKCD08466
Size:96 Wells
Price: $850.00
SKU
OKCD08466
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KLF2 ELISA Kit (Rat) (OKCD08466) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KLF2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KLF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KLF2 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level KLF2. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level KLF2 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
KLF2 Microplate96 Wells (12 x 8 Well strips)
KLF2 Lyophilized Standard2
100X KLF2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.068ng/mL
Gene SymbolKLF2
Gene Full NameKruppel-like factor 2
Alias SymbolsLklf
NCBI Gene Id306330
Protein NameKrueppel-like factor 2
Description of TargetTranscription factor that binds to the caccc box in the promoter of target genes such as hbb/beta globin or nov and activates their transcription.
Uniprot IDQ9ET58
Protein Accession #NP_001007685.1
Nucleotide Accession #NM_001007684.1
Write Your Own Review
You're reviewing:KLF2 ELISA Kit (Rat) (OKCD08466)
Your Rating
We found other products you might like!